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DVEB05313: 100 reactions- FAM, Cy5DVEB05353: 50 reactions- FAM, Cy5
Detection of the 16S rRNA gene of Aerococcus viridansBackground: Aerococcus viridans is a Grampositive bacterium causing gaffkemia, a fatal lobster disease, and it has been associated with septicemia in sea turtles. Aerococcus viridans has also been isolated from the milk of cows with subclinical mastitis. It can also cause different human infections. Description: BactoReal® Kit Aerococcus viridans uses real-time PCR for the detection of the 16S rRNA gene of Aerococcus viridans. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG). Product Features: - Amplification and detection: apxIVA gene of Actinobacillus pleuropneumoniae - Real-time PCR with rapid hot-start Taq DNA polymerase - ROX™ dye as passive reference - Internal Positive Control System to exclude false-negative results - Optimized to handle PCR inhibitors - PCR- platforms: runs on all established standard real-time PCR- platforms - Harmonized thermal profiles to run RNA and DNA samples simultaneously - Kits format: 100 reactions or 50 reactions - Channel Target: FAM; Channel IPC: VIC/HEX