BactoReal® Kit Coxiella burnetii

Detection of the IS1111 insertion sequence of Coxiella burnetii

Background: Coxiella burnetii (C. burnetii) is a Gramnegative, obligate intracellular, zoonotic bacterial pathogen, which is the causative agent of Q fever (Coxiellosis). The disease is spread through inhalation of a spore-like cell variant or close contact with body fluids. Cattle, sheep and goats represent the main animal reservoir.Seropositivity of ruminants for C. burnetii is associated with abortion, stillbirth and fertility problems. The high resistance to standard disinfectants and environmental influences renders disease control difficult.

Description: BactoReal® Kit Coxiella burnetii uses real-time PCR for the detection of the IS1111 insertion sequence of Coxiella burnetii. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: IS1111 of Coxiella burnetii
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: VIC/HEX