REAL TIME PCR DETECTION TEST KIT FOOD SAFETY Escherichia coli (EPEC VTEC and EHEC)

Detection of E. coli pathogenic genes (stx1 , stx2 and eae) in food products, animal feedstuff and environmental samples. Pre-enrichment according to ISO 16654:2001 (recommended). For use in real time instruments with FAM and ROX channels.

BACKGROUND: Escherichia coli, can cause enteric/diarrhoegenic or extra-intestinal infections in humans. Enteric E. coli that cause disease can present different virulence determinants, corresponding to different pathotypes. These include the verocytotoxigenic E. coli (VTEC), comprising the enterohaemorrhagic E. coli (EHEC), and the enteropathogenic E. coli (EPEC). VTEC are characterized by the production of verocytoxins (Vtx), encoded by vtx1 or vtx2 genes, (also known as Shiga-like toxins - Stx - corresponding to stx1 and stx2 genes). EHEC are a subset of VTEC, that in addition to the Vtx-encoding genes carries the attaching and effacing gene eae (intimin-coding) and have the ability to cause attaching and effacing lesions in hosts. EPEC carry the eae gene but do not produce Vtx. The E. coli strains, specially EHEC (including the O157:H7 and O157:H- serotypes), are recognized as major pathogens of foodborne disease in humans. They are mostly transmitted through undercooked minced beef, inadequately pasteurized milk, cold sandwiches, sprouts and vegetables.
The need for rapid, accurate and sensitive methods for the detection of these E. coli strains is a major food safety issue. Since conventional microbiological methods for their detection and identification are time-consuming. Today, there are approved methods for PCR base detection of pathogenic genes of Escherichia coli unique DNA sequences, in particular using real time PCR and specific fluorescent probes.

PRODUCT DESCRIPTION: REAL TIME DETECTION KIT E. coli uses real-time PCR for the detection of the pathogenic genes of E. coli (eae, Vtx1 and Vtx2) in a simple, reliable, and rapid procedure. The assay is based on 5’ nuclease real time PCR reactions to amplify a unique genomic sequence in the target microorganism. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal control (IC).

PRODUCT FEATURES:
-Amplification and detection: eae, Vtx1 and Vtx2 genes of E. coli EPEC, VTEC and EHEC
-Real-time PCR with rapid hot-start Taq DNA polymerase
-Internal Control to exclude false-negative results
-PCR- platforms: runs on all established standard real-time PCR- platforms
-Harmonized thermal profiles to run with other BIOPREMIER kits simultaneously
-Channel Pathogen: FAM, Channel IC: ROX
-Available formats: Kits with 50 reactions for each target gene or 5 reactions for each target gene