Real Time Pcr Detection Test Kit Water Legionella Spp.

Detection of Legionella spp. in water samples. Water samples shall be concentrated according to ISO 11731. The kit is suitable for use in real time instruments with FAM and ROX detection channels.

BACKGROUND: Legionnaires’ disease is the most severe form of infection, caused by Legionella strains, which includes pneumonia, and the fatality rate can approach 50% in immunocompromised patients. Outbreaks of Legionnaires’ disease have been worldwide traced to a wide variety of environmental water sources such as cooling towers, hot tubs, showerheads, whirlpools and spas, and public fountains. Over the last few years, the reported incidence of legionellosis has steadily increased. Commonly used method for environmental surveillance of Legionella spp. is the standard culture technique, but it has several limitations as it requires selective media and prolonged incubation periods. Recently, rapid and sensitive alternative methods have been found to be attractive alternatives to the conventional culture method for the detection of slow-growing and fastidious bacteria such as Legionella spp. These methods are PCR based methods, in particular using real time PCR and specific fluorescent probes, that allow the rapid detection of Legionella spp. in water samples.

PRODUCT DESCRIPTION: REAL TIME DETECTION KIT Legionella spp. uses real-time PCR for the detection of Legionella spp. in a simple, reliable, and rapid procedure. The assay is based on 5’ nuclease real time PCR reactions to amplify a unique genomic sequence in the target microorganism. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal control (IC).

PRODUCT FEATURES:
-Amplification and detection of Legionella spp.
-Real-time PCR with rapid hot-start Taq DNA polymerase
-Internal Control to exclude false-negative results
-PCR- platforms: runs on all established standard real-time PCR- platforms
-Harmonized thermal profiles to run with other BIOPREMIER kits simultaneously
-Channel Pathogen: FAM, Channel IC: ROX
-Available formats: Kits with 100 or 10 reactions