Rt - Real Time Pcr Detection Test Kit Supreme Hlvd / Lcv / Cancv

Detection of Hop latent viroid (HLVd), Lettuce Chlorosis Virus (LCV), and Cannabis Cryptic Virus (CanCV) in Cannabis or other plant samples. The BIOPREMIER SUPREME RT-PCR Detection Kit HLVd / LCV / CanCV combines a reverse transcriptase with a multiplex qPCR assay to simultaneously detect HLVd, LCV, CanCV and an Internal Control. The kit is suitable for use in real time instruments with FAM, Cy5, HEX/VIC and ROX/Texas Red detection channels.

BACKGROUND:
Hop latent viroid (HLVd) is a single-stranded, circular infectious RNA that is dependent on its host plant’s metabolism for replication. HLVd infection occurs worldwide in hops (Humulus lupulus) but it can also infect the cannabis plant. The HLVd-infected plants don’t show obvious symptoms of infection but instead grow shorter with smaller leaves, which reduce the production of certain cultivars. HLVd is usually spread via infected tools or through cloning (cuttings taken from an infected mother).
Lettuce Chlorosis Virus (LCV) is a single-stranded RNA, which is usually found in some plants host such as lettuce, cannabis and hemp plants. The young LCV-infected plants don’t show obvious symptoms of infection until a few weeks. After that, the infection doesn't kill the plants, but their yield is significantly affected. The plant will produce smaller flowers, brittle, yellowish and sometimes necrotic. LCV can be transmitted from plant to plant via pests, water or cloning (cuttings taken from an infected mother).
Cannabis Cryptic Virus (CanCV) is a double-stranded RNA virus and infects plant species of the Cannabaceae. The effects of CanCV on its hosts are not yet well understood, and it is frequently found in asymptomatic plants.
Because symptoms of these viruses are not always obvious, regularly screening by qPCR is the key to isolating the infected plants from their growing area to prevent the spread of the virus throughout the productive field.

PRODUCT DESCRIPTION: SUPREME REAL TIME DETECTION KIT HLVd / LCV / CanCV uses Reverse Transcriptase (RT) real-time PCR for the detection of HLVd, LCV, CanCV in a simple, reliable, and rapid procedure. The assay combines a reverse transcriptase with a multiplex qPCR assay based on 5’ nuclease real time PCR reactions to amplify a unique genomic sequence of each target. Our carefully designed primers and probe ensure the highest sensitivity and specificity. The kit consists of an assay mix for detecting the targets, a positive control, and internal control (IC).To minimize PCR cross-contamination, the Kit contains dUTP and uracil-N glycosylase (UDG).

PRODUCT FEATURES:
-Amplification and detection of HLVd, LCV, CanCV (simultaneously)
-Transcription ensured by the enzyme Reverse transcriptase
-Real-time PCR with rapid hot-start Taq DNA polymerase
-Internal Control to exclude false-negative results
-Optimized to handle PCR inhibitors, containing UDG enzyme
-PCR- platforms: runs on all established standard real-time PCR- platforms
-Channel Target: FAM, HEX/VIC and ROX/Texas Red
-Channel IC: Cy5
-Available formats: Kits with 100 or 500 reactions